Virologica Sinica
December 4 2024
Identification of mutations in viral proteins involved in cell adaptation using a reverse genetic system of the live attenuated hepatitis A virus vaccine H2 strain
Xiu-Li Yana,b, Jian Lib,c, Qing-Qing Mab, Hong-Jiang Wangd, Lin Lib,c, Hui Zhaob, Cheng-Feng Qinb,✉, Xiao-Feng Lia,b,✉
a School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui, 230032, China;
b Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, 100071, China;
c School of Medicine, Tsinghua University, Beijing, 100084, China;
d Department of Research, The Chinese People's Liberation Army Strategic Support Force Medical Center, Beijing, 100101, China
10.1016/j.virs.2024.08.004
The live attenuated hepatitis A virus vaccine H2 strain was developed by passaging a wild-type H2w isolate in cell cultures. Currently, the mechanism underlying its attenuation phenotype remain largely unknown. In this study, we generated a full-length infectious cDNA clone of the H2 strain using in-fusion techniques. The recovered H2 strain (H2ic) from the cDNA clone exhibited an efficient replication in both the hepatoma cell line Huh7.5.1 and the 2BS cell line used for vaccine production, similar to the parental H2 strain.
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